Table 2 List of reagents and equipment
From: Live imaging and analysis of postnatal mouse retinal development
Name of the reagent | Company | Catalogue number | Comments (optional) |
|---|---|---|---|
Dissection and in vitro electroporation | Â | ||
Hank’s Balanced Salt Solution (-) Calcium Chloride, (-) Magnesium Chloride | GIBCO | 14175 | Denoted HBSS (-/-) in text |
Hank’s Balanced Salt Solution (+) Calcium Chloride, (+) Magnesium Chloride | GIBCO | 14025 | Denoted HBSS (+/+) in text |
Dulbecco’s Modified Eagle Medium (DMEM) high-glucose | GIBCO | 11960 |  |
Neurobasal Medium (-) glutamine | GIBCO | 21103 | Â |
100 μl electroporation cuvette | Custom assembled |  |  |
ECM-830 square wave electroporator | BTX | Â | Â |
no. 55 Dumostar forceps | Fine Science Tools | 11295-51 | Â |
no. 5 Dumont forceps | Fine Science Tools | 11251-30 | Â |
Dissecting microscope | Nikon | SMZ-800 | Â |
Light source | Fiber-Lite | M1-150 | Â |
100x20 mm culture dish | Sarstedt | 83.1802 | Â |
6-well culture dish | Starstedt | 83.1839 | Â |
Polycarbonate Track-Etch Membranes | Whatman | 110606 | 25mm, 0.2 um |
B27 supplement | GIBCO | 17504044 | Â |
Glutamax | GIBCO | 35050-061 | Â |
1000 μl wide-bore pipette tips | Axygen | T-1005-WB-C-R |  |
200 μl pipette tips |  |  | Generic brand |
20 up pipette tips | Â | Â | Generic brand |
Explant preparation for histology | Â | Â | Â |
HBSS (-/-) | GIBCO | 14175 | Â |
4% paraformaldehyde in 0.1M PBS | Electron Microscopy Sciences | 157-8 | 8% stock PFA |
0.1M PBS | Â | Â | Â |
0.1M PBS with 30% sucrose | Â | Â | Â |
O.C.T. Compound | Tissue-Tek | 4583 | Â |
Adhesive coated slides | Newcomer Supply | 5070 | 75x25x1.0 mm |