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Table 2 List of reagents and equipment

From: Live imaging and analysis of postnatal mouse retinal development

Name of the reagent Company Catalogue number Comments (optional)
Dissection and in vitro electroporation  
Hank’s Balanced Salt Solution (-) Calcium Chloride, (-) Magnesium Chloride GIBCO 14175 Denoted HBSS (-/-) in text
Hank’s Balanced Salt Solution (+) Calcium Chloride, (+) Magnesium Chloride GIBCO 14025 Denoted HBSS (+/+) in text
Dulbecco’s Modified Eagle Medium (DMEM) high-glucose GIBCO 11960  
Neurobasal Medium (-) glutamine GIBCO 21103  
100 μl electroporation cuvette Custom assembled   
ECM-830 square wave electroporator BTX   
no. 55 Dumostar forceps Fine Science Tools 11295-51  
no. 5 Dumont forceps Fine Science Tools 11251-30  
Dissecting microscope Nikon SMZ-800  
Light source Fiber-Lite M1-150  
100x20 mm culture dish Sarstedt 83.1802  
6-well culture dish Starstedt 83.1839  
Polycarbonate Track-Etch Membranes Whatman 110606 25mm, 0.2 um
B27 supplement GIBCO 17504044  
Glutamax GIBCO 35050-061  
1000 μl wide-bore pipette tips Axygen T-1005-WB-C-R  
200 μl pipette tips    Generic brand
20 up pipette tips    Generic brand
Explant preparation for histology    
HBSS (-/-) GIBCO 14175  
4% paraformaldehyde in 0.1M PBS Electron Microscopy Sciences 157-8 8% stock PFA
0.1M PBS    
0.1M PBS with 30% sucrose    
O.C.T. Compound Tissue-Tek 4583  
Adhesive coated slides Newcomer Supply 5070 75x25x1.0 mm