Table 2 List of reagents and equipment
From: Live imaging and analysis of postnatal mouse retinal development
| Name of the reagent | Company | Catalogue number | Comments (optional) |
|---|---|---|---|
| Dissection and in vitro electroporation | |||
| Hank’s Balanced Salt Solution (-) Calcium Chloride, (-) Magnesium Chloride | GIBCO | 14175 | Denoted HBSS (-/-) in text |
| Hank’s Balanced Salt Solution (+) Calcium Chloride, (+) Magnesium Chloride | GIBCO | 14025 | Denoted HBSS (+/+) in text |
| Dulbecco’s Modified Eagle Medium (DMEM) high-glucose | GIBCO | 11960 | |
| Neurobasal Medium (-) glutamine | GIBCO | 21103 | |
| 100 μl electroporation cuvette | Custom assembled | ||
| ECM-830 square wave electroporator | BTX | ||
| no. 55 Dumostar forceps | Fine Science Tools | 11295-51 | |
| no. 5 Dumont forceps | Fine Science Tools | 11251-30 | |
| Dissecting microscope | Nikon | SMZ-800 | |
| Light source | Fiber-Lite | M1-150 | |
| 100x20 mm culture dish | Sarstedt | 83.1802 | |
| 6-well culture dish | Starstedt | 83.1839 | |
| Polycarbonate Track-Etch Membranes | Whatman | 110606 | 25mm, 0.2 um |
| B27 supplement | GIBCO | 17504044 | |
| Glutamax | GIBCO | 35050-061 | |
| 1000 μl wide-bore pipette tips | Axygen | T-1005-WB-C-R | |
| 200 μl pipette tips | Generic brand | ||
| 20 up pipette tips | Generic brand | ||
| Explant preparation for histology | |||
| HBSS (-/-) | GIBCO | 14175 | |
| 4% paraformaldehyde in 0.1M PBS | Electron Microscopy Sciences | 157-8 | 8% stock PFA |
| 0.1M PBS | |||
| 0.1M PBS with 30% sucrose | |||
| O.C.T. Compound | Tissue-Tek | 4583 | |
| Adhesive coated slides | Newcomer Supply | 5070 | 75x25x1.0 mm |
