Figure 3

PKCδ expression is highest within the nervous system at E13.5. A-D, at embryonic stage E13.5, whole mount staining allowed for the detection of LacZ signal in skin (ski) in homozygous but not heterozygous embryos. Choroid plexus (cho), neural tube (neu), caudal region of the medulla oblongata (cmo), inferior ganglion of glossofaringeal (XI) nerve (inf), trigeminal (V) ganglion (trg), and dorsal root ganglia (drg) all showed novel β-galactosidase activity in whole embryos. E-M, 15 μm sagittal sections were obtained from LacZ stained embryos. Reporter activity observed in most cells of dorsal root ganglia (drg), trigeminal (V) ganglion (trg), inferior ganglion of glossofaringeal (XI) nerve (inf), vestibulocochlear ganglion (ves) and neural tube confirmed the signal observed in LacZ-stained whole embryos. Also, LacZ reporter activity was detected at cartilage primordium at limbs (pre), the loop of midgut within physiological umbilical hernia (loo), dorsal part of tongue (ton), and lower border of nasal septum (sep). Areas labelled as * could be detected only via LacZ staining and thus were not reported. N and O, at E13.5, PKCδ deficient mouse sections (δ−/−) were used as negative controls for immunohistochestristry (A and B). P-Y, at E13.5, antibodies to PKCδ confirmed LacZ staining at dorsal root ganglia (drg), trigeminal (V) ganglion (trg), inferior ganglion of glossofaringeal (XI) nerve (inf), vestibulocochlear ganglion (ves), loop of midgut within physiological umbilical hernia (loo), dorsal part of tongue (ton) and lower border of nasal septum (sep). Cross sections allowed for immunodetection of PKCδ in cartilage primordium at limbs (pre), metanephros (met) and wall and mucosal lining of the stomach (sto). In addition, antibody staining was detected in the atrium of the heart (atr).