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Figure 2 | BMC Developmental Biology

Figure 2

From: Expression of phosphatase of regenerating liver family genes during embryogenesis: an evolutionary developmental analysis among Drosophila, amphioxus, and zebrafish

Figure 2

Drosophila PRL is enriched in developing mid-gut and the ventral nerve cord in embryogenesis. (A-D) Whole mount in situ hybridization using antisense probes against Drosophila PRL. (A) dmPRL was ubiquitously expressed in stage 5 embryos. (B) At stage 9, dmPRL was expressed in developing anterior and posterior mid-guts. (C) At stage 13, dmPRL was expressed in developing anterior and posterior mid-guts together with VNC. (D) At stage 16, the expression of dmPRL was significant in procephalon. (E-H) Whole mount immunostaining of Drosophila PRL during embryogenesis. At stage 5 (E) and stage 9 (F), DmPRL was ubiquitously expressed. (G) At stage 13, dmPRL was expressed in developing anterior and posterior mid-guts together with VNC. (H) At stage 15, the expression of dmPRL was enriched in VNC. (I-K) dmPRL (I) and axon marker BP102 (J) were colocalized (K) in VNC of stage 15 embryos. Abbreviations: am, anterior mid-gut; pm, posterior mid-gut; VNC, ventral nerve cord; mg, mid-gut; pc, procephalon.

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