Figure 5

Multiple genes transfer and tissue specific expression via foamy viruses. A. EGFP and DsRED expression is seen in A1 cells transduced with mixture of EGFP and DsRED foamy viruses. A’. untransduced A1 cells. B. Cross section of tail showing spinal cord and surrounding nerve tracts. Tail blastema was transduced with mixture of FV containing DsRed and EGFP FV particles. Colocalization is seen between EGFP and DsRed fluorescent signal.C-F. Muscle-specific expression was achieved when FV vector particles harboring the Xenopus CarAct:EGFP cassette were injected into the blastema at day 5 after amputation of a 3 cm larvae. C. Cross section of limb showing EGFP expression in muscle tissue. D. MHC immunostaining of section to highlight muscle cells/fibers. E. PAX7 immunostaining of same section to delineate satellite cells. F. Overlay to show that GFP signal is exclusive of PAX7+ satellite cells. Scale bars: A,A’: 200 μm, B: 20 μm, C-F: 50 μm.