Figure 2
From: Expression pattern of Protein Kinase C ε during mouse embryogenesis
PKCϵ expression in whole mount embryos from E10.5 to E12.5. A -C, At E10.5, trigeminal neural crest (tnc), facio-acoustic neural crest complex (fac), neural tube in caudal region of the tail (nt), cephalic mesenchyme (cm), dorsal root ganglia (drg), and roof of the hindbrain (rh) show reporter activity. D, At stage E11.5, staining of the trigeminal (V) ganglion (trg), facio-acoustic (VII-VIII) ganglion complex (fac), dorsal root ganglia (drg), as well as interior ganglion of vagus (X) nerve (ivn) became all prominently LacZ stained. E-H, At E12.5, novel LacZ activity in the caudal part of the medula oblongata (cmo) and developing kidney (dk) can be detected. Embryos at this stage also show clear LacZ signal in blood vessels (fig. H), which was weakly detectable from E9.5. I, Western blotting showing expression of PKCϵ in wild type mouse embryonic endothelial cells (MEECs). PKCϵ deficient MEEC were used as negative control and VEGFR2 was used as endothelial marker.