Figure 1
From: Expression pattern of Protein Kinase C ε during mouse embryogenesis
PKCϵ expression at E8.5 and E9.5. A-C, lack of Lac Z signal due to endogenous β-galactosidase was confirmed on wild type embryos (controls). D, at E8.5, X-Gal staining is detected in the yolk sac and primitive heart (ys and ph, respectively). E-G, at E9.5, developing heart (hea), somites (som) and Rathke’s pouch (Rp) showed LacZ signal. H-M, Antibody staining of 5 μm E.9.5 embryo sections confirmed the expression of PKCϵ in somites, Rathke’s pouch and heart. In the latter domain, immunoreactivity was detected at the walls of the primitive ventricle (ven), atrium (atr) and truncus arteriosus (tru). E9.5 PKCϵ deficient embryo sections were used as a negative control for the antibody (J).