Figure 4

Summary of experimental results on meiotic spindle structure following injection of the 14-3-3η morpholino and control conditions. As described in Methods, oocytes were held in prophase I meiotic arrest for 24 hours, allowed to mature in vitro for 13 hours and then processed for immunofluorescence and confocal microscopy to examine the spindle structure. (A) For each condition, the number of cells with a normal spindle, deformed spindle or no spindle is represented graphically as a percentage of the total number of cells examined. Light grey, spindle absent; dark grey, deformed spindle; black, normal spindle. The number of cells with absent, deformed or normal spindles out of the total number of cells studied for each injection condition is indicated alongside the corresponding percentage bars. Representative images of cells displaying the three categories are presented in Figure 5 and 6. (B) Fisher’s Exact test comparing the experimental group (14-3-3η morpholino injection) and combined controls for the absence of spindles or presence of at least some spindle. (C) Fisher’s Exact test comparing the experimental group (14-3-3η morpholino injection) and combined controls for deformed or normal spindle. MO, morpholino oligonucleotide.