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Figure 1 | BMC Developmental Biology

Figure 1

From: Identifying targets of the Sox domain protein Dichaete in the Drosophila CNS via targeted expression of dominant negative proteins

Figure 1

Dominant negative proteins mimic Dichaete phenotypes. A-F) BP102 staining reveals the ventral nerve cord of stage 16 embryos. All ventral views with anterior to the top. A) sim-GAL4;UAS-D; B) sim-GAL4;UAS-DΔHMG; C) sim-GAL4;UAS-DEnRep; D) pros-GAL4;UAS-D; E) pros-GAL4;UAS-DΔHMG; F) pros-GAL4;UAS-DEnRep. Arrows indicate breaks in the longitudinal connectives, arrowheads indicate collapse of commissures. G-L) FasII staining revealing the major longitudinal fascicles G) Wild type; H) Dr72/Df(3L)fz-GS1a; I) sim-GAL4;UAS-DΔHMG; J) sim-GAL4;UAS-DEnRep; K) sim-GAL4;UAS-mSox2ΔHMG; L) sim-GAL4;UAS-mSox2EnRep. M-P) Anti-Slit staining to reveal the midline glia. M and N) Wild type at stage 13 (M) and 15 (N). O and P) sim-GAL4;UAS-DΔHMG at stage 13 (O) and 15 (P). M and O are ventral views with anterior to the left, N and P are lateral views with anterior to the left. Grey arrowheads indicate loss of Slit expression.

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