Figure 1

Dominant negative proteins mimic Dichaete phenotypes. A-F) BP102 staining reveals the ventral nerve cord of stage 16 embryos. All ventral views with anterior to the top. A) sim-GAL4;UAS-D; B) sim-GAL4;UAS-D^ΔHMG; C) sim-GAL4;UAS-D^EnRep; D) pros-GAL4;UAS-D; E) pros-GAL4;UAS-D^ΔHMG; F) pros-GAL4;UAS-D^EnRep. Arrows indicate breaks in the longitudinal connectives, arrowheads indicate collapse of commissures. G-L) FasII staining revealing the major longitudinal fascicles G) Wild type; H) D^r72/Df(3L)fz-GS1a; I) sim-GAL4;UAS-D^ΔHMG; J) sim-GAL4;UAS-D^EnRep; K) sim-GAL4;UAS-mSox2^ΔHMG; L) sim-GAL4;UAS-mSox2^EnRep. M-P) Anti-Slit staining to reveal the midline glia. M and N) Wild type at stage 13 (M) and 15 (N). O and P) sim-GAL4;UAS-D^ΔHMG at stage 13 (O) and 15 (P). M and O are ventral views with anterior to the left, N and P are lateral views with anterior to the left. Grey arrowheads indicate loss of Slit expression.