Figure 4

Immunofluorescence staining for endothelial-associated markers 72 h post-induction on matrigel. hMSC-TERT, hADMSCs, hADSSCs and hNFSSCs were induced for 7 days and then were plated on matrigel-coated wells. Expression of endothelial associated markers (CD31, VE-cadherin, eNOS, VEGF165 and vWF) was assesses using immunofluorescence microscopy. 4,6-Diamidino-2 phenylindole (DAPI) was used to counter stain for cell nuclei (images are shown at magnification of 10×). Notably, hNFSSCs exhibited tremendous tightly packed capillary tube-like structures. Lower right panel is a close-up for vWF staining shown at 20× magnification.