Figure 5
Loss-of-function Pdcd4 mutations result in an increase in the number of cells with a spherical spectrosome. (A-F) Immunofluorescence images showing single projections of confocal Z-stacks of germaria from 5-day-old females. Genotypes are listed in the bottom right corner of each panel. Immunostaining with anti-Hts (green channel in A-C and red channel in D-F) decorated the spectrosomes (ss) and fusomes (fs). Anti-Vasa (red in A-C) decorated germ cells, and the GFP protein trap Pdcd4G93 decorated the apical tips of the germaria. Scale bars are 10 μm. (A) Representative wild-type germarium showing the typical four cells with spherical spectrosomes. (B-F) Representative germaria from Pdcd4 mutants showing an increased number of cells with spherical spectrosomes. (G) Frequency histogram showing the mean number of cells containing spherical spectrosomes ± standard error for the indicated genotypes. Cells numbers were scored by scanning up and down through confocal Z-stacks. A minimum of 38 germaria from at least 19 females were scored for each genotype. ** indicates a p-value less than or equal to 1 × 10-16 for a comparison between the number of spectrosome-containing cells in the respective mutants vs wild-type.