Figure 4

Sequence similarity and genetic organization of Drosophila Programmed cell death 4 ( Pdcd4 , formally called CG10990 ). (A) Alignment of Pdcd4 protein sequences from H. sapiens (human) and D. melanogaster. The alignment of identical residues and chemically similar residues are indicated by asterisks and paired dots respectively. The extent of the two MA-3 protein domains are indicated by blue (N-terminal) and red (C-terminal) shading. Yellow shading indicates the extent of highly similar sequences outside the MA-3 domains. (B) Organization of the Pdcd4 locus. Exons are indicated by rectangles, the transcription start site is indicated by an arrow, open reading frame is shaded yellow, and sequences encoding the two MA-3 domains are shaded blue and red (corresponding to the shading in A). The locations of the transgene insertions are indicated by triangles. The CG10990-GFP protein trap line G00093 is referred to as Pdcd4^G93. The Pdcd4¹ allele was induced by FRT mediated recombination between the insertions PBac{WH}CG10990^f06108 and P{XP}CG10990^d04016 and the region deleted in this mutation is indicated by a dashed line.