3D-FISH analysis of embryonic nuclei in mouse highlights several abrupt changes of nuclear organization during preimplantation development

Table 4 Characterization of NPBs/nucleoli in mouse embryonic nuclei

Stage	No. of nuclei	No. of NPBs/nucleoli	Mean no. of NPBs/nucleoli per nuclei	Relative volume of NPBs/nucleoli (% of nuclear volume)	Median volumes of NPBs/nucleoli (μm³) [1^st quartile; 3^rd quartile]
Early 2-cell	72	887	12.2 ± 3.90 ^a	14.9 ± 3.93 ^ab	28.7 ^a
Early 2-cell	72	887	12.2 ± 3.90 ^a	14.9 ± 3.93 ^ab	[16.0; 50.2]
Late 2-cell	211	1959	9.9 ± 3.07 ^b	15.6 ± 3.68 ^a	41.5 ^b
Late 2-cell	211	1959	9.9 ± 3.07 ^b	15.6 ± 3.68 ^a	[16.9; 78.9]
Early 4-cell	100	1091	10.9 ± 4.19 ^ab	14.1 ± 4.47 ^bc	12.1 ^cd
Early 4-cell	100	1091	10.9 ± 4.19 ^ab	14.1 ± 4.47 ^bc	[6.2; 24.4]
Late 4-cell	87	275	2.7 ± 1.9 ^c	14.3 ± 3.06 ^c	64.5 ^e
Late 4-cell	87	275	2.7 ± 1.9 ^c	14.3 ± 3.06 ^c	[13.4; 203.9]
Early 8-cell	89	364	4.1 ± 2.62 ^d	13.3 ± 2.32 ^cd	13.4 ^cf
Early 8-cell	89	364	4.1 ± 2.62 ^d	13.3 ± 2.32 ^cd	[5.5; 58.3]
Late 8-cell	184	883	4.7 ± 2.65 ^d	12.6 ± 2.39 ^d	11.1 ^d
Late 8-cell	184	883	4.7 ± 2.65 ^d	12.6 ± 2.39 ^d	[4.3; 33.0]
16-cell	158	283	2.2 ± 2.13 ^e	10.8 ± 2.93 ^e	26.1 ^af
16-cell	158	283	2.2 ± 2.13 ^e	10.8 ± 2.93 ^e	[4.9; 81.0]
32-cell	117	220	1.9 ± 1.41 ^e	9.6 ± 2.87 ^f	19.6 ^cd
					[3.4; 50.9]

Within each column, values with different subscripts are significantly different at p < 0.01.

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