Figure 2

Ectopic expression of GFP-CAP restores aggregation defects of aca^- cells. (A) Aggregation competent AX2 and aca^-cells and corresponding transformants expressing GFP-CAP were starved for 6 h in Soerensen phosphate buffer. Cells were fixed with methanol at -20°C and immunostained with mAb act1 specific for actin. Bar, 10 μm. (B) Time course of agglutination. 1 × 10⁷ cells/ml were starved in Soerensen phosphate buffer, and at the indicated times the optical density (OD) as a measure for cell agglutination was determined at 600 nm using a spectrophotometer. Error bars represent the standard deviation. Statistics were performed using the Student's t-test and the P value < 0.05 is indicated with *.