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Figure 1 | BMC Developmental Biology

Figure 1

From: Ectopic expression of cyclase associated protein CAP restores the streaming and aggregation defects of adenylyl cyclase a deficient Dictyostelium discoideumcells

Figure 1

Expression of CAP and GFP fusions of CAP or its domains in aca- cells. 5 × 105 cells were lysed in 2 × SDS sample buffer and separated on 12% SDS-PAGE. Immunoblots show the expression of endogenous CAP and GFP-fusions of full length or N- or C-terminal domains of CAP with or without the proline rich regions in aca- cells. Endogenous CAP (50 kDa) and GFP-CAP (~80 kDa) were detected with mAb 230-18-8 (A) or with GFP specific mAb K3-184-2 (B) that detected only GFP-tagged proteins or with CAP C-terminus specific mAb 223-445-1 (C), that detected both endogenous and GFP-CAP. The lower band in B (aca- -GFP-CAP) is a degradation product. (D) Actin (~42 kDa) detected with mAb act1 served as loading control. (E) GFP-fusions of CAP in aca- cells were detected with mAb K3-184-2. (F), actin control. In A-D an AX2 lysate was included to allow the comparison for endogenous CAP in the wild type. A-D, lanes 1, AX2 whole cell lysate; lanes 2, aca- whole cell lysate; lanes 3, whole cell lysate from aca- cells expressing full length GFP-tagged CAP.

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BMC Developmental Biology

ISSN: 1471-213X

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