Figure 4

Quantification of integrins released from the border cells during migration. In the confocal micrographs of ( A) wild-type ( w¹¹¹⁸), (B) CalpB⁵⁰⁵, (C) slbo-Gal4, ( D) slbo-Gal4 >UAS-CalpBRNAi, ( E) slbo-Gal4 >UAS-rheaRNAi, ( F) slbo-Gal4 >UAS-rheaRNAi; CalpB⁵⁰⁵ egg chambers, the areas of egg chambers around and behind the border cells are labeled with phalloidin-TRITC (red) for actin, with anti-β-PS integrin (green), and with DAPI (blue) for nuclei. In the inserts, the area directly behind the border cells is magnified. The movement of the border cells proceeds from the left to the right, and the released integrins left behind the cells appear as discrete spots (labeled by arrowheads) at the left sides of the images. Representative pictures of 20–23 independent experiments are shown. The calculated average numbers of integrin spots at the rear of the border cells per egg chamber, and the corresponding number of investigated egg chambers (n) are indicated by the diagrams at the right side of each panel. Note that only the spots falling into the cells’ pathway were scored, and groups of closely situated spots were counted as a single “spot”. Bars indicate 100 μm.