Figure 7

Histamine receptor 1 antagonist (125 μM chlorpheniramine) did not affect caspase activity in pre-competent larvae. Caspase activity was analyzed using FAM-VAD-FMK, a fluorescently tagged caspase inhibitor. Overall fluorescence was measured in the arms tips of competent larvae. Histamine (1 μM), Histamine receptor 1 antagonist (125 μM chlorpheniramine) and histamine (1 μM) plus Histamine receptor 1 antagonist (125 μM chlorpheniramine) treatment had no significant effect on caspase activity. Scale bars: 20 μm.