Wild type expression data for folate pathway transcripts. A) RT-PCR analysis of 11 embryonic stages within the first 72 hours of development indicates that genes involved in the folate pathway are generally maternally loaded transcripts that are expressed throughout early development. B) in situ hybridization for these 16 genes at 24 hpf shows that many are globally expressed with enrichment in anterior neural tissues including tectonic and retinal staining. Specific somatic staining is seen in mat1a, mat2aa, mat2ab, and mthfr. YSL staining is also observed in mat1a and mthfd1b. Embryos in lateral view.