Figure 4

Knock-down of individual desmosomal cadherins causes developmental defects. The effects of morpholino knockdown of zfDsc and zfDsga as recorded at 24 hpf. As phenotypes for both morpholino knock-downs were very similar, representative images from their relative phenotypic classifications are shown in E-P. For comparison, WT animals are shown in A-D. Quantification of phenotypic distribution from morpholino knockdown of zfDsc and zfDsga are shown in Q and R, respectively. (N.B. Embryos that showed epiboly defects (see text and Figure 5) generally did not progress beyond 10 hpf and are therefore recorded as dead in this figure.) Quantification of standard control morpholino induced phenotypes and zfDsc/zfDsga knock-down embryos that have been partially rescued with corresponding Dsc1/Dsg2a mRNA are shown in S and T, respectively. Scale bars in A, E, I and M = 40 μm; other scale bars = 20 μm.