Figure 5
From: In vivo role of different domains and of phosphorylation in the transcription factor Nkx2-1
Thyroid differentiation in E18 PM/PM embryos. Transversal sections of thyroid gland and trachea of wild type (A-E) and PM/PM (F-J) E18.5 embryos were stained with hematoxylin and eosin (A and F), anti-Nkx2-1(B and G), anti Pax8 (C and H), anti Tg (D and I) and anti-NIS (E and J )antibodies. The follicular organization of the thyroid gland (panel A, arrows) appears impaired and empty follicules are visible (panel F, arrowheads) in mutant thyroid. In PM/PM embryos, follicular cells express Nkx2-1, Pax8 and Tg at levels comparable with those of wild type embryos while the expression of NIS appears increased in the mutant ones(F vs J). Original magnification ×630 for panels A and F; ×100 for the other panels.