Figure 7
Immunofluorescent and lectin labeling of the planarian testes. (A-C) Single confocal optical sections showing either immuno or lectin staining (magenta). Nuclei are counterstained with DAPI (grey). (A) Antibody labeling with anti-tubulin δ2 (delta tubulin). Insets show magnified views of flagella (magenta) on round spermatids (dashed box) and sperm (solid box). (B) Peanut agglutinin (PNA) staining in the testes. Top panel shows a confocal section of a testis with labeling of spermatogonial cells (sg), bottom panel shows a more ventral confocal section of the same testis with labeling of spermatids (st). (C) Lens culinaris agglutinin (LCA) labels the membranes in and around the testes. Scale bars: 20 μm.