Figure 6

Immunofluorescent and lectin labeling of the planarian reproductive system. (A-H) Confocal images of immuno or lectin staining in red/magenta/green, DAPI in grey. A) Oviducts (od) and sperm ducts (sd) are labeled by anti-tubulin δ2 (delta tubulin). B) The tuba and oviducts are labeled by anti-muscle (muscle), anti-phosphotyrosine (p-tyr) and anti-carbonic anhydrase (ca). Oocytes (oo) are found in the ovary anterior to the tuba, sperm (s) collect in the tuba. C) Bends in the oviducts (yellow arrows). D) The bursal canal (bc) connects to the oviducts. E) The bursal canal is also visualized by anti-phosphotyrosine. F) Lectin Peanut agglutinin (PNA) labels the bursal canal and glands around the copulatory apparatus, lectin Ricinus communis agglutinin (RCA) and Erythrina cristagalli lectin (ECL) also label glands around the compulatory apparatus. G) Seminal vesicles containing sperm are suspended in a network of muscle fibers. H) The penis papilla is labeled by anti-phosphotyrosine. Scale bars: (A, C-H) 50 μm; (B) 20 μm.