Figure 3
grn and tsp-1 are expressed in accessory reproductive organs. (A-C, top panel) Whole mount view of two-color FISH showing expression of grn (green) and tsp-1 (magenta) in sexual S. mediterranea. Anterior of animal is to the left. (A-C, middle and bottom panels) Single confocal optical sections showing expression of grn (green) and/or tsp-1 (magenta). Nuclei are counterstained with DAPI (grey). (A) grn is expressed in the seminal vesicles and sperm ducts. (B) tsp-1 is expressed in glands around the copulatory bursa and bursal canal. The tsp-1-expressing cells are interspersed between testis lobules near the copulatory apparatus. (C) Two-color FISH showing distinct populations of cells expressing either grn or tsp-1. Sperm duct (sd); seminal vesicles (sv); bursa (b); bursal canal (bc); testes (t). Scale bars: (A-C, top panel) 500 μm; (A-C, middle and bottom panels) 100 μm.