Figure 6

Comparison of the Distributions of Migration Velocity Between FMB- and HB-NCCs. E9.5 P0-Cre/CAG-CAT-EGFP embryos were used as samples to discriminate NCCs from the other cell types. We isolated cells from the region rostral or caudal to the MHB. We named the rostral NCC population FMB-NCC and the caudal NCC population HB-NCC. Then we seeded the isolated cells to collagen-coated plates for 4 hours of time-lapse observation. We measured the "mean velocity through all the frames (Va)" of both populations (A (n = 201) and B (n = 417)). The two populations have similar Va mode value categories (FMB-NCC: 16-18 μm/hours, HB-NCC: 18-20 μm/hours) and were also similar in minimum Va (FMB-NCC: 9.6 μm/hours, HB-NCC: 11.2 μm/hours). Big differences were observed between the populations in maximum Va (FMB-NCC: 45.5 μm/hours, HB: 94.5 μm/hours), mean Va (FMB-NCC: 20.4 μm/hours, HB-NCC: 29.1 μm/hours), and standard deviation (SD) of Va (FMB-NCC: 5.4 μm/hours, HB-NCC: 13.1 μm/hours).