Figure 8

P10 flatmount retinas labeled with anti-GFAP (red) and GS isolectin (green). A complete and remodeled primary or superficial vasculature was observed in the WT mouse (A) and the deep vascular plexus was forming at this stage (B). A panoramic image of the Lama1^nmf223 retina with only GS isolectin labeling (C) demonstrated the abnormal persistence of the VHP, growth of vessels into vitreous from the peripapillary retina and the formation of a complex capillary network within the retina. Double labeling with anti-GFAP and GS isolectin (D) showed continued association of astrocytes with the hyaloid vessels. There appeared to be two layers of astrocytes within the vitreous, one which ensheathed the persistent VHP and one below the vessels on the surface of the retina. Also evident is branching from the intravitreal vessels (arrow) into the retina (D) to form the intraretinal plexus (E). Asterisks mark the optic nerve head. Scale bars indicate (A-C: 100 μm; D, E: 50 μm).