Figure 7
Cross sections of whole eyes from Lama1nmf223 P7 mice were labeled with GS isolectin (green), anti-pan laminin (red), and DAPI (blue). A panoramic composite of a representative Lama1nmf223 retina demonstrated the migration of blood vessels out of the retina towards the VHP (paired arrows) and across the vitreal surface of the ILM (A). Higher magnification of this area (B, C) demonstrated the presence of a laminin-positive membrane-like structure surrounding the exiting blood vessels (arrows) and exiting through the ILM (opposing open arrowheads). Labeling of an adjacent section (D) with anti-PDGFRα (light blue) along with GS isolectin (green), anti-pan laminin (red), and DAPI (blue) showed astrocytes within retina (upward solid arrowhead) and on the vitreal side (downward arrowhead) of the ILM (open arrowhead). Higher magnification (E) showed an astrocyte (solid arrowhead) migrating from the retina to vitreous through the ILM (open arrowhead). Scale bars indicate (A: 50 μm; B, C, E: 10 μm; D: 20 μm).