Figure 6

mRNA in situ hybridization of TGIF2 during spermatogenesis in tammar adult testis. Each panel shows a seminiferous tubule at different stages of the spermatogenic cycle indicated by the steps 1 to 14 (S1 to S14) [28]. All images shown were hybridized with antisense probe (purple blue) and counterstained with nuclear fast red. Images boxed on the right bottom are the high resolution pictures from the corresponding part highlighted with dashed boxes. TGIF2 mRNA was detected in the cytoplasm of early round spermatids (A, step 2) and round spermatids before transforming to elongated spermatids (B, step 5). Staining was also observed in the cytoplasm of elongated spermatids and spermatocytes (C-E, step7-12). Image F indicates that there was no staining on the mature spermatozoa with arrows showing the sperm head (bottom arrow) and sperm tail (top arrow) (step 14). SG, spermatogonia; SP, spermatocytes; RS, round spermatids; ES, Elongating/elongated spermatids; SZ, spermatozoa; SC, Sertoli cells. Scale bars = 100 μm.