Figure 4

Expression pattern of tammar TGIF1 and TGIF2 in various tissues by RT-PCR. A) Expression of the TGIF1 &TGIF2 gene was assessed in various embryonic tissues. Both TGIF1 and TGIF2 were widely expressed in all examined tissues; B) Expression of the TGIF1 &TGIF2 during gonadal development from the bipotential gonad stage (day 25 of gestation-d25), sexual differentiation stages (days 2-3 post partum for the testis and days 7-8 for the ovary) and at later stages of development (up to day 44 post partum). Tammar TGIF1 and TGIF2 were detected at all stages; C). Expression of TGIF1 &TGIF2 was examined in various adult tissues. TGIF1 was broadly expressed in most tissues and TGIF2 was specifically expressed in the ovary, testis and spleen. PCR for 18S (100 bp) acted as a positive control and "-ve" indicates template free negative control reactions.