Transdifferentiation from cornea to lens in Xenopus laevis depends on BMP signalling and involves upregulation of Wnt signalling

Table 4 Q-rtPCR validation of 13 genes from the RAG list.

Rank in array	Gene name	Lens (L/S)	CLT (R/S)
		qPCR	qPCR	array
3	Tcf7	0.6	5.7	7.8
10	Crop	0.7	1.4	3.3
11	Nipsnap1 (sim)	0.1	176.3	8.5
12	Taf1b	1.0	8.0	10.2
15	Mybph	0.0	0.2	52.1
31	Pdik1l-b	1.1	10.3	12.3
38	Gnai2	5.5	56.1	6.3
41	Tcf4	0.0	4.4	4.3
45	Mmp-11	1.8	100.0	2.4
56	Creld1	0.0	1.6	5.2
71	Amph	24.2	74.6	2.9
479	Mmp14	0.1	3.4	2.3
1391	Lmo4	4.11	2.2	1.4

Bold numbers indicate upregulation of more than 1.5 fold, numbers in italics indicate down regulation and normal font indicates no significant change. L/S shows the relative (fold higher) expression in lens when compared to sham operated corneas. Likewise R/S shows relative expression in corneas undergoing CLT when compared to sham operated control corneas.

ISSN: 1471-213X