Figure 5
Expression of human LHX1, PAX8 and HNF1B in animal caps. Animal caps of LHX1 and/or PAX8 (250 pg alone or 125 pg each) or HNF1B (150 pg) injected embryos were cultured in Steinberg's solution for three hours and analysed after four days by whole-mount immunostaining using a mixture of the antibodies 3G8 and 4A6. Activin A (ActA, 10 ng/ml) and retinoic acid (RA, 10-4M) were added as given. (A) and (B) The pronephric tissue induction in animal caps treated as indicated on the left was scored using the categories given in Figure 4A. The number of animal caps (N) is given.