Table 4 Sequences of primer specific for target genes

MyoD

Forward

5'-GAC GGC TCT CTC TGC TCC-3'

Reverse

5'-AAG TGT GCG TGC TCC TCC-3'

Myogenin

Forward

5'-GAG CGC GAT CTC CGC TAC AGA GG-3'

Reverse

5'-CTG GCT TGT GGC AGC CCA GG-3'

MCK

Forward

5'-CAA TAA GCT TCG CGA TAA GGA G-3'

Reverse

5'-GAT GGG ATC AAA CAG GTC CTT G-3'

Id1

Forward

5'-ACG ACA TGA ACG GCT GCT ACT-3'

Reverse

5'-GCT CAC TTT GCG GTT CTG G-3'

Runx2

Forward

5'-TTT AGG GCG CAT TCC TCA TC-3'

Reverse

5'-TGT CCT TGT GGA TTA AAA GGA CTT G-3'

Osteocalcin

Forward

5'-TGC TTG TGA CGA GCT ATC AG-3'

Reverse

5'-GAG GAC AGG GAG GAT CAA GT-3'

ALP

Forward

5'-CAC GGG CAC CAT GAA GGA AAA G-3'

Reverse

5'-TGG CGC AGG GGC ACA GGA GAC T-3'

Collagen II

Forward

5'-ACT GGT AAG TGG GGC AAG AC-3'

Reverse

5'-CCA CAC CAA ATT CCT GTT CA-3'

Collagen X

Forward

5'-TGG GTA GGC CTG TAT AAA GAA CGG-3'

Reverse

5'-CAT GGG AGC CAC TAG GAA TCC TGA GA-3'

Cyclin D1

Forward

5'-AGG CGG ATG AGA ACA AGC AGA-3'

Reverse

5'-CAG GCT TGA CTC CAG AAG GG-3'

CDK4

Forward

5'-ACG CCT GTG GTG GTT ACG CT-3'

Reverse

5'-CCA TCT CTG GCA CCA CTG AC-3'

BMP-2

Forward

5'-GTG GTG GAA GTG GCC CAC TT-3'

Reverse

5'-CTG TTT GTG TTT GGC TTG ACG-3'

BMP-4

Forward

5'-TCC TGG TAA CCG AAT GCT GAT-3'

Reverse

5'-GCT GCT GAG GTT GAA GAG GAA-3'

GAPDH

Forward

5'-GAT GCT GGT GCT GAG TAT GTC G-3'

Reverse

5'-GTG GTG CAG GAT GCA TTG CTG A-3'