Figure 6
From: PAPC and the Wnt5a/Ror2 pathway control the invagination of the otic placode in Xenopus
xPAPC depletion is rescued by ca RhoA but not by disheveled. (A, B) Coinjection of different disheveled constructs, shown in (A). (B) 500 pg RNA were single side coinjected with xPAPC Mo at 16-cell stage in every one of the two adjacent ventral and dorsal cells in the animal part. At stage 26 the embryos were examined via Tbx2 ISH. The data are normalized relating to uninjected wildtype embryos. None of the constructs showed a reconstitution, so neither the Dsh-coupled PCP, nor canonical Wnt signaling pathway is involved in PAPC function. (C) Small GTPases Rac1 and RhoA were coinjected with xPAPC Mo as dominant negative (dn) or constitutive active (ca) constructs. The injection was carried out as described in (B). To further reduce secondary effects the injection was carried out with DNA. Reconstitution can be achieved by coinjection of 5 pg ca RhoA. Ca, constitutive active; Dn, dominant negative; Dsh, disheveled; FL, full length; Mo, antisense morpholino; n, number of embryos. ** = p < 0,01.