Representative total proteome gels of P. vexillosa. Pre: pre-competent larvae, Com: competent larvae, Juv: newly metamorphosed juveniles. (A) After 2-DE, the gels were stained with the total protein stain Sypro Ruby. (B) Number of protein spots reproducibly detected in the three developmental stages. (C) Number of up-regulated and down-regulated total protein spots. Differentially expressed spots showed a greater than two-fold change and a significant difference between stages (Student's t-test (p < 0.01, n = 3).