Figure 3

HDAC inhibition leads to increased levels of acetylated histones and H3K4me2 on the XNr-1 gene. (A) Schematic showing the structure of the Xenopus Nr-1 gene. Light gray boxes represent the protein-coding and the dark gray box represents the promoter region (adapted from [53]). Intronic regions 1 and 2 are indicated. Red and blue arrows represent the primer set used for qPCR reaction for the promoter and intronic region, respectively. (A1) The red lines indicate the sequence of the XNr-1 promoter region used to design the primer set for qPCR reaction. (A2) The regions underlined show the sequence used for primer set design. In purple are highlighted the FAST binding domains as in [53] and the black CATTTG indicates two putative Mad binding sites. (B) Chromatin isolated from embryos exposed to NaB from stage 1-7 and allowed to develop until stage 21 in 0.1X MMR was used for ChIP with anti-acetyl H4, anti-acetyl H3, anti-H3K4me2 and rabbit IgG (Control) followed by qPCR analysis against the promoter region (gray bar) and intronic region (black bar) of XNr-1. The levels of acetylated H4 and H3 and H3K4me2 were increased only on the intronic region (black bars).