Figure 5

Germ layer segregation in 'ciuffo' embryos. Sections through the posterior protrusions of 24hpf 'ciuffo' embryos stained with characteristic germ layer markers show that neuroectoderm specific markers. islet1 (A) and krox20 (B) are expressed in the outermost layer of the protrusion. The endodermal marker gata5 (C) is expressed in the innermost layers, whereas the mesodermal marker myoD (B) can be observed in between. Sections were made of 24 hpf ich embryos that had been injected with βcatMO1 and βcatMO2, hybridized as whole mounts with the indicated probes.