Figure 3

Embryos inhibited in both BMP signaling and organizer function form neuroectoderm with correct AP pattern at the end of gastrulation and this patterning can be modulated by similar mechanisms as in wild-type embryos. Single in situ hybridization with cyp26 (A,D,G,J,M,P,S,V,Y) or hoxb1b (B,E,H,K,NQ,T,W,Z) probes or double in situ hybridization with both probes (C,F,I,L,O,R,U,X,A') is shown for wild-type embryos (A-C), untreated ich embryos (D-F), or ich embryos treated with bmp2bMO (G-I), the two β-cat MOs (J-L), bmp2bMO plus the two β-cat MOs (M-O), or ich embryos injected with antivin mRNA (P-R), antivin mRNA plus bmp2bMO (S-U), SU5402 (V-X), or SU5402 plus bmp2bMO (Y-A'). Wild-type embryos [A-C] are shown in dorsal views, while ich embryos are shown in lateral views. The neuroectodermal and tailbud expression domains of cyp26 in wild-type embryos are marked with arrow and star, respectively. All embryos are at ~100% epiboly.