Figure 8
Tie2Cre-mediated eYFP expression indicates BrdU-positive cells are endothelial. E12.5 VYS mesoderm tissues from Tie2Cre+/0·Srff/f embryos are virtually devoid of SRF protein yet continue to proliferate. Double-label immunofluorescence images of E12.5 VYS from Tie2Cre+/0·Srff/f·ROSA26R-eYFP+/+ embryos. Tissues were stained for GFP (green), BrdU (red), and DNA (blue); monochrome images show anti-BrdU (B) and anti-GFP (C) staining in isolation. Arrows mark individual BrdU-positive cells in VYS mesoderm also expressing eYFP. Magnification = 400×, scale bar = 20 μm; Ed = endoderm, Md = mesoderm.