Figure 5
ECM in Tie2Cre+/0·Srff/f VYS tissue lacks FN1. FN1 contributes to ECM surrounding developing vessels and is critical for signaling in VYS tissues. Double-label immunofluorescence images of VYS tissue from wild type (A, C) and SRF-null (B, D) embryos at E12.5 (A, B) and E10.5 (C, D). Tissues were stained for E-Cad (green), FN1 (red), and DNA (blue). Arrows/arrowheads mark FN1 expression. Magnification = 200×, scale bar = 50 μm; Ed = endoderm, Md = mesoderm.