Figure 1

Conditional mouse EC-N1ICD and EC-Rbpj-KO transgene constructs. (A) Upon crossing of mice that carry the Rosa^Notch transgene to a transgenic mouse that expresses CRE in the endothelia, recombination removes the neomycin cassette and induces expression of the Notch1 intracellular domain only in endothelial cells. (B) Upon crossing of mice that carry the Rbpj^f transgene [22] to a transgenic mouse that expresses Cre in the endothelia, recombination will remove the neomycin cassette along with exons 6 and 7, which encode for the DNA binding domains of RBPJ, abrogating the promoter activity of RBPJ only in endothelial cells.