Figure 6

PI3K regulation: Analysis of microarray data and expression of a subset of PI3K regulated genes. A) Scatter graph showing the signal intensity of probe sets. B) Functional analysis of negatively and positively regulated genes by PI3K using Ingenuity Pathway Analysis software. C) Representative northern blot analyses of genes regulated by PI3K originally identified by DNA microarray analysis. D) qRT-PCR analysis of genes regulated by PI3K originally identified by DNA microarray analysis. See Tables 1 and 2 for a list and description of the mRNAs investigated. Rcho-1 trophoblast stem cells were cultured under differentiating conditions with exposure to vehicle (0.1% DMSO; D+V) or LY294002 (10 μM; D+LY). Student's t-tests (*P < 0.05).