Figure 8
Analysis of cbt promoter, expression and requirement in cell death- induced regeneration. (A) Graphical representation of one AP1 site and two E-boxes that are evolutionarily conserved in the promoter of cbt and detailed sequences of each binding site in different Drosophila species. This picture was produced using the UCSC genome browser [76]. (B-E) In situ hybridizations in wing imaginal discs. (B) Wild-type disc showing ubiquitous cbt expression. (C) Wild-type disc with cbt sense RNA probe as a control. (D) cbt expression in en > hepCA disc. Notice upregulation of cbt in the posterior compartment. (E) Confocal section of a wing disc after 24 hours of regeneration. cbt is overexpressed near the wound. (F-J) Fluorescent in situ hybridization (blue) in disc in which cell death has been induced; nuclei are stained red. (F) Basal confocal section of a wing disc after 10 hours of rpr induction in the salm domain. The dotted line indicates the cell death domain. cbt expression is constant throughout the disc. (G) High-magnification view of the white square in F. (H) Apical confocal section of a wing disc after 10 hours of rpr induction in the salm domain. Regenerating cells in this domain show a high level of cbt expression. (I) High-magnification view of the white square in H, showing increased expression of cbt. (J) Cross-section through a stack of images (from apical to basal) at the level of the yellow line in F. Scale bars = 50 μm.