Figure 5

Knock-down of burs transcripts by RNAi impedes progression to the pharate adult stage. (A) Development of Act5C>burs RNAi animals proceeds until the pharate adult stage, when all progeny die trapped within the puparium. (B) BURS-IR comparisons of Act5C>burs RNAi larvae and driver-less UAS-burs RNAi control larvae confirms that ubiquitous expression of burs RNAi severely limits the expression of bursicon at the NMJ, as quantified by fluorescence intensity. For each data point, larval NMJs (n = 16) from two animals were observed. Representative 'RNAi' and 'Control' NMJs accompany the data points (in lower panels). Error bars indicate +/- SEM.