Table 3 Oligonucleotide primers used for qualitative real-time PCR analysis
Gene (accession number) | Primer | Sequence | Position* |
|---|---|---|---|
ovSOLD1 | Forward | 5'--GGAGGCACCTGCCAGACTCA--3' | 128--147 |
(AB297496) | Reverse | 5'--AAAGCGTGCCATCTTCGTAG--3' | 197--178 |
caSOLD1 | Forward | 5'--GGAGGCACCTGCCAGACTCA--3' | 128--147 |
(AB297497) | Reverse | 5'--AAAACATGCCATCTTCGTAG--3' | 197--178 |
ovGAPDH | Forward | 5'--GCCATCACCATCTTCCAGGA--3' | 81--100 |
(AF030943) | Reverse | 5'--CCACGTACTCAGCACCAGCA--3' | 150--131 |
caGAPDH | Forward | 5'--GCCATCACCATCTTCCAGGA--3' | 115--134 |
(AJ431207) | Reverse | 5'--CCACGTACTCAGCACCAGCA--3' | 184--165 |
boNXN | Forward | 5'--TCCTAGTGGAGTCCTACCGGAA--3' | 715--736 |
(NM_001102136) | Reverse | 5'--CCTGTCCGCACTAACAAAGATG--3' | 788--767 |
boBCL2L13 | Forward | 5'--TCGGGAATGTACACTGGAGACC--3' | 592--613 |
(NM_001078082) | Reverse | 5'--GTAGCAAAATCAGAGGCACCAA--3' | 665--644 |
boGAPDH | Forward | 5'--AAGGCCATCACCATCTTCCA--3' | 178--197 |
(U85042) | Reverse | 5'--CCACTACATACTCAGCACCAGCAT--3' | 253--230 |