Figure 3
Whole-mount embryo in situ hybridization (ISH) using a FITC-conjugated granzyme G oligonucleotide probe at different stages of normal mouse development. (A to C) One-cell stage mouse embryos. (D to F) Two-cell stage mouse embryos. (G to I) Four-cell stage mouse embryos. (J to K) Eight-cell stage mouse embryos. The left panels (A, D, G, and J) show embryos photographed under phase contrast imaging. The middle panels (B, E, H, and K) show embryos stained with Hoechst 33342 for DNA localization (blue) under fluorescence microscope observation. The right panels (C, F, I, and L) show embryos hybridized with a granzyme G oligonucleotide probe (green) under confocal microscopic observation. Scale bar: 30 μm.