Chuzhoi mutants exhibit minor defects in neural crest cell distribution. (A-L) Wholemount in situ hybridisation for Sox10 expression at E10.5 in wild-type (A-C), heterozygous (D-F) and chuzhoi homozygous mutant (G-L) embryos. Wild-type and heterozygous embryos showed normal cranial (V, VII/VIII, IX, Xj (jugular) and Xn (nodose)) and dorsal root ganglia (drg). Chuzhoi homozygous mutants exhibit abnormally shaped trigeminal ganglia (V), and apparent fusion of the ninth (IX) and tenth (Xn) cranial ganglia. The distrubution of neural crest cells in the spinal region also appears abnormal, with disturbance of the normal V-shaped arrangement of stained cells in the dorsal root ganglia. B, E, H, K are higher power views of the branchial arches (K is left side of embryo shown in J); C, F, I, L are higher power views of the dorsal root ganglia.