Figure 5

Duf/Kirre is mislocalized in Vrp1^f06715mutants. (A-C) Embryos stained for Duf/Kirre (red) and Alk (green). (A) Duf/Kirre accumulates in punctate foci in Vrp1^f06715mutant embryos (arrow). No Duf/Kirre expression can be detected at this stage in controls (see C). (B) Close up of VM in a Vrp1^f06715embryo in which accumulation of Duf/Kirre can be detected (arrow). (C) In wild type embryos Duf/Kirre expression is low and is not visible (arrow indicates absence of Duf/Kirre accumulations). (D) twip-GFP-actin-Vrp1^f06715embryo, in which a GFP-actin fusion protein is expressed under the control of the twist promoter [25]. After VM fusion Duf/Kirre accumulation in the VM is visible (arrowheads), but these show no obvious build-up of actin-GFP, indicating that the Duf/Kirre accumulations do not contain elevated actin expression.