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Figure 4 | BMC Developmental Biology

Figure 4

From: Generation of human induced pluripotent stem cells by simple transient transfection of plasmid DNA encoding reprogramming factors

Figure 4

Differentiation of iPSK3 into hepatocyte-like cells. A) Micrograph showing the epithelial-like organization of iPSK3 upon hepatic differentiation. Higher resolution (inset) showing a binucleated cell. Scale bar = 100 μm. B) The hepatocyte-like cells derived from iPSK3 metabolized and transported 5-(and 6)-carboxy-2'-7'-dichlorofluorescein diacetate between cells (high-resolution inset, white arrow), indicating the presence of functional bile transporters. C) Immunocytochemistry revealed the presence of proteins commonly associated with hepatocytes including, FoxA2, Hnf4α, E-cadherin, α-fetoprotein and Albumin. Scale bar = 100 μm.

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