Figure 2

The expression of glial and neuronal specific markers in the developing retina. Retina tissue from chicken embryos were harvested at various time points during development, sectioned, and stained with antibodies Vimentin (A-F), Pax6 (G-L), and NeuN (M-R). While these markers are not cell-type specific, they label proteins that are involved in retina development. Vimentin labels radial glia in retina at early embryonic stages and Müller glia cells in retina at late embryonic stages. The Vimentin labeling resulted in the characteristic striated banding that stretched across the layers of the retina. Pax6 labels horizontal, amacrine, and ganglion cells. NeuN is a marker of early neurons and in the retina labels amacrine and ganglion cells. ONBL, outer neuroblastic layer; INBL, inner neuroblastic layer; ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer. Scale bar = 40 μm.