Figure 4

mig-13 and ceh-13 interact in controlling Q cell migration. A, Fluorescent images showing mec-7::gfp expression in ceh-13(-) and mig-13(-) single mutant animal, as well as in a ceh-13(-); mig-13(-) double mutant animal. The positions of AVM and PVM are indicated by white arrows. B, Relative final positions of AVM in mig-13 and ceh-13 deficient animals. The length of the X axis corresponds to the relative length of the animals. The left is at anterior. Vertical dotted lines indicate the birthplace of the Q cells. At least 100 animals were scored for each strain. C, Schemes showing the migration pattern of AVM and PVM in ceh-13(-) and mig-13(-) single mutant backgrounds, as well as in ceh-13(-); mig-13(-) double mutant background. D, mig-13 expression requires ceh-13 activity. White arrows point to the pharyngeal-intestinal valve cells. The vast majority of ceh-13(-) mutants failed to or weakly express mig-13 (93%, N = 133). Mutant animals were captured with the same or even a longer exposure time than was applied for the wild-type background.