Figure 4

Characterization of Mpl-positive cells. (A) Co-localization of Mpl and the pan-neuronal marker NeuN. Almost all Mpl-positive cells (green) are NeuN+ neurons (red) with the exception of cerebellar white matter. (B) Double-labelling of sections for Mpl and NeuroD, an early marker of granule cells. Mpl (green) does not overlap with NeuroD (red) in the cerebellum or in the hippocampus. (C) Expression of Mpl and the astrocyte marker GFAP in different brain regions at P0, P7 and in the adult brain. There is no overlap between Mpl (green) and GFAP (red). cp - cortical plate; egl - external granule layer; gcl - granule cell layer; h - hippocampus; wm - white matter. Scale bars: 20 μm in A and B, 50 μm in C.