Figure 4
From: Deletion of Irs2 causes reduced kidney size in mice: role for inhibition of GSK3β?
Increased PKB/Akt expression in Irs2-/- mouse kidney. Twenty μg of kidney protein lysates from 5-6 wk Irs2+/+, Irs2+/- and Irs2-/- 5-6 wk male (A) and female (B) groups were separated on 10% SDS-PAGE and probed by Western blotting with anti-PKB/Akt. GAPDH was used as a loading control. Band intensities were calculated using Scion Image software. The intensity ratios of PKB/GAPDH are shown. ** p < 0.01.